Protein Biochemistry Lab — 부산대학교 화학과

Research

01 — Degradation Biochemistry

Lysosomal Protein Degradation

리소좀 내 단백질 분해 기전을 분자 수준에서 규명합니다. 카텝신 단백질 분해효소의 활성 조절, 기질 특이성, 그리고 세포 항상성 유지에서의 역할을 생화학적·구조생물학적 접근법으로 연구합니다.

We investigate the molecular mechanisms of lysosomal protein degradation. Using biochemical and structural biology approaches, we study the activity regulation, substrate specificity, and roles of cathepsin proteases in cellular homeostasis.

02 — Molecular Transport

Organelle Ion Channels and Transporters

리소좀을 비롯한 세포 소기관막에 위치한 이온 채널 및 수송체 단백질의 분자적 작동 기전을 연구합니다. 이러한 작동 기전을 바탕으로, 이들이 소기관의 이온·분자 항상성과 생리적 기능에 미치는 영향을 생물리학적·구조생화학적 방법론을 통해 규명합니다.

We investigate the molecular mechanisms underlying the operation of ion channels and transporter proteins in the membranes of lysosomes and other organelles. Building on these mechanistic insights, we elucidate how these proteins shape organellar ion and molecular homeostasis and govern their physiological functions, using biophysical and structural biochemistry approaches.

Members

Principal Investigator

SeCheol Oh

Assistant Professor
Department of Chemistry
Pusan National University

Biography

2025–PresentAssistant Professor, Department of Chemistry, Pusan National University
2017–2025Research Fellow / Senior Research Scientist, Memorial Sloan Kettering Cancer Center, Structural Biology Program
2010–2017Ph.D., Weill Cornell Medicine, New York — Biochemistry, Cell Biology & Molecular Biology
2007–2010Lecturer, Korea Military Academy, Seoul
2005–2007M.S., KAIST — Chemistry
2001–2005B.S., KAIST — Chemistry

Graduate Students

Hye-won Cho

M.S. Student

Publications

At Pusan National University

2026

Biosynthesis and Characterization of Antibacterial Fe-Ag Bimetallic Nanocomposites mediated by Lacticaseibacillus paracasei-derived Cell-Free Supernatants

S Kashyap, B Konwar, S Oh, K Kim

J. Environ. Chem. Eng., 14(1), 120910

2026.2

2025

Biogenic Selenium Nanoparticles from Lactiplantibacillus plantarum as a Potent Antimicrobial Agent Against Methicillin-Resistant Staphylococcus aureus

G Kim, S Oh, K Kim

Pharmaceutics, 18(1), 14

2025.12

Before Pusan National University

2024

Discovery of selective inhibitors for the lysosomal Parkinson's disease channel TMEM175

S Oh, J Lee, HJ Choi, S Kim, V Sapuru, M Kim and RK Hite

J. Am. Chem. Soc., 146(33), 23230–23239

2024.8

2022

Mechanism of 4-aminopyridine inhibition of the lysosomal channel TMEM175

S Oh, R Stix, W Zhou, JD Faraldo-Gomez and RK Hite

Proc. Natl. Acad. Sci. USA, 119(44), e2208882119

2022.10

2022

Differential ion dehydration energetics explains selectivity in the non-canonical lysosomal K⁺ channel

S Oh*, F Marinelli*, W Zhou*, J Lee, HJ Choi, M Kim, JD Faraldo-Gomez and RK Hite (*equal contribution)

eLife, 11, e75122

2022.5

2022

Structure of the Wilson disease copper transporter ATP7B

M Bitter*, S Oh*, Z Deng, S Rahman, RK Hite and P Yuan (*equal contribution)

Sci. Adv., 8, eabl5508

2022.3

2020

Gating and selectivity mechanism for the lysosomal K⁺ channel TMEM175

S Oh, N Paknejad and RK Hite

eLife, 9, e53430

2020.3

2019

Cryo-EM structures of the DCPIB-inhibited volume-regulated anion channel LRRC8A in lipid nanodiscs

DM Kern, S Oh, RK Hite and SG Brohawn

eLife, 8, e42636

2019.2

2018

Kinetic mechanism of coupled binding in sodium-aspartate symporter GltPh

S Oh and O Boudker

eLife, 7, e37291

2018.9

2015

Isothermal titration calorimetry of ion-coupled membrane transporters

O Boudker and S Oh

Methods, 76, 171–182

2015.4

2014

Coupled ion binding and structural transition along the transport cycle of glutamate transporters

G Verdon*, S Oh*, R Serio and O Boudker (*equal contribution)

eLife, 3, e02283

2014.5

2013

Binding thermodynamics of a glutamate transporter homolog

N Reyes, S Oh and O Boudker

Nat. Struct. Mol. Biol., 20, 634–640

2013.4

2007

Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist eritoran

HM Kim, BS Park, JI Kim, SE Kim, J Lee, S Oh, P Enkhbayar, N Matsushima, H Lee, OJ Yoo and Jie-Oh Lee

Cell, 130(5), 906–917

2007.9

2007

Structural diversity of the hagfish variable lymphocyte receptors

HM Kim*, S Oh*, KJ Lim, J Kasamatsu, JY Heo, BS Park, H Lee, OJ Yoo, M Kasahara and Jie-Oh Lee (*equal contribution)

J. Biol. Chem., 282(9), 6726–6732

2007.3

Full list on Google Scholar ↗

Protein
Biochemistry
Lab

Research

Lysosomal Protein Degradation

Organelle Ion Channels and Transporters

Members

Publications

Biosynthesis and Characterization of Antibacterial Fe-Ag Bimetallic Nanocomposites mediated by Lacticaseibacillus paracasei-derived Cell-Free Supernatants

Biogenic Selenium Nanoparticles from Lactiplantibacillus plantarum as a Potent Antimicrobial Agent Against Methicillin-Resistant Staphylococcus aureus

Discovery of selective inhibitors for the lysosomal Parkinson's disease channel TMEM175

Mechanism of 4-aminopyridine inhibition of the lysosomal channel TMEM175

Differential ion dehydration energetics explains selectivity in the non-canonical lysosomal K⁺ channel

Structure of the Wilson disease copper transporter ATP7B

Gating and selectivity mechanism for the lysosomal K⁺ channel TMEM175

Cryo-EM structures of the DCPIB-inhibited volume-regulated anion channel LRRC8A in lipid nanodiscs

Kinetic mechanism of coupled binding in sodium-aspartate symporter GltPh

Isothermal titration calorimetry of ion-coupled membrane transporters

Coupled ion binding and structural transition along the transport cycle of glutamate transporters

Binding thermodynamics of a glutamate transporter homolog

Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist eritoran

Structural diversity of the hagfish variable lymphocyte receptors

News

Contact

ProteinBiochemistryLab

Research

Lysosomal Protein Degradation

Organelle Ion Channels and Transporters

Members

Publications

Biosynthesis and Characterization of Antibacterial Fe-Ag Bimetallic Nanocomposites mediated by Lacticaseibacillus paracasei-derived Cell-Free Supernatants

Biogenic Selenium Nanoparticles from Lactiplantibacillus plantarum as a Potent Antimicrobial Agent Against Methicillin-Resistant Staphylococcus aureus

Discovery of selective inhibitors for the lysosomal Parkinson's disease channel TMEM175

Mechanism of 4-aminopyridine inhibition of the lysosomal channel TMEM175

Differential ion dehydration energetics explains selectivity in the non-canonical lysosomal K⁺ channel

Structure of the Wilson disease copper transporter ATP7B

Gating and selectivity mechanism for the lysosomal K⁺ channel TMEM175

Cryo-EM structures of the DCPIB-inhibited volume-regulated anion channel LRRC8A in lipid nanodiscs

Kinetic mechanism of coupled binding in sodium-aspartate symporter GltPh

Isothermal titration calorimetry of ion-coupled membrane transporters

Coupled ion binding and structural transition along the transport cycle of glutamate transporters

Binding thermodynamics of a glutamate transporter homolog

Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist eritoran

Structural diversity of the hagfish variable lymphocyte receptors

News

Contact

Protein
Biochemistry
Lab